ascorbic acid (Thermo Fisher)

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Thermo Fisher ascorbic acid
Ascorbic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ascorbic+acid/L-(%2B)-Ascorbic+acid%2C+98%2B%25/pm42354430-215-44-46
Average 94 stars, based on 1 article reviews

ascorbic acid - by Bioz Stars, 2026-10

94/100 stars

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Modification:

Article Title: Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.
Article Snippet: 8-arm polyethylene glycol acrylate (8-arm PEG acrylate, 10 kDa; Jenkem Technology, Plano, TX, USA), sodium alginate with an M/G ratio of 0.6 (Protanal® LF 10/60, IFF Pharma Solutions, Wilmington, DE, USA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Thermo Fisher Scientific, Waltham, MA, USA), N-hydroxysuccinimide (NHS; Tokyo Chemical Industry, Tokyo, Japan), 2-(N-morpholino) ethanesulfonic acid buffer (MES, 1.0 M, pH 7.0; Thermo Fisher Scientific), and adipic acid dihydrazide (AAD; Tokyo Chemical Industry). .. Dithiothreitol (DTT) and ethylene glycol bis(mercaptoacetate) (EGBMA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, 1:1), fetal bovine serum (FBS), penicillin–streptomycin (PS), L-glutamine, Dulbecco’s phosphate-buffered saline (DPBS), and 0.05% trypsin–EDTA, Rat tail collagen type I, The LIVE/DEAD Viability/Cytotoxicity Kit, 4′,6-diamidino-2-phenylindole (DAPI), and 4% paraformaldehyde (PFA), ascorbic acid (Thermo Fisher Scientific). .. Dexamethasone (Gibco, Grand Island, NY, USA), insulin–transferrin–selenium (ITS (1X), Sigma-Aldrich, Saint Louis, MO, USA), D1 mouse mesenchymal stem cells (ATCC, passage 11).

Saline:

other:

Article Title: Antimicrobial and regenerative copper-doped hybrid biomaterials for bone infection treatment.
Article Snippet: Phosphate buffer saline (PBS, pH 7.4) was purchased from Merck (Germany).

Article Title: A Rapid 3D Melanoma–Skin Organoid for High-Throughput Assessment of Tumor Dynamics and Drug Response
Article Snippet: On day 8, cultures were transitioned to the air–liquid interface (ALI) and supplemented with 50 μg/mL ascorbic acid (Thermo Fisher Scientific Inc.; Waltham, MA, USA ref.J66601.06) to initiate epidermal differentiation and support barrier formation over the following 14 days.

Construct:

Article Title: Characterization and development of a gelatin/elastin methacrylamide-based bioink for creating a 3D bioprinted human skin model.
Article Snippet: Three-dimensional (3D) human skin constructs are gaining increasing relevance important in regenerative research, drug testing and the cosmetics industry.. They offer great potential to overcome the anatomical and physiological limitations of animal models, yet current models are often limited by high costs, batch-to-batch variability, and mechanical properties that poorly replicate native skin.. In particular, discrepancies in elasticity and tensile strength compared to the natural extracellular matrix (ECM) of skin remain a major challenge.

Cell Culture:

Article Title: Functional maturation of enteric neurons derived from human induced pluripotent stem cells.
Article Snippet: On D15 of differentiation, cells were dissociated with Accutase and plated on 6-well plastic plates, glass coverslip dishes (Ibidi) or 8-well chamber slides (ThermoFisher) coated with poly-L-ornithine (PO; 15 μg/mL; Sigma Aldrich), laminin (LN; 2 μg/mL; R&D Systems) and fibronectin (FN; 2 μg/mL; Corning) at a density of 100,000 cells per cm 2 . .. Cells were cultured in NM containing N2 supplement (10 μL/mL), B27 supplement minus Vitamin A (20 μL/mL), GlutaMax (10 μL/mL), MEM NEAA (10 μL/mL) and Ascorbic Acid (100 μM; ThermoFisher Scientific) and glial derived neurotrophic factor (GDNF; 10 ng/mL; Peprotech), with media changes every 2 days. ..

Article Title: Nanofiber-based platform for quantitative analysis of human oligodendrocyte ensheathment with pharmacological perturbations.
Article Snippet: .. On day 0, cells were cultured in DMEM/F12 containing 0.5% N2 supplement, 0.5% B27 supplement lacking vitamin A, GlutaMAX, antibiotics, 1 μM SAG, 200 μM ascorbic acid, 10 ng/mL IGF-I (Thermo Fisher), 10 ng/mL PDGF-AA (Thermo Fisher), 10 ng/mL 3, 3 ′ , 5-triiodo-L-thyronine sodium salt (T3) (FujiFilm), 0.1% trace elements cocktail solution (0.17 ng/mL MnSO 4 ∙ H 2 O, 140 ng/mL Na 2 SiO 3 ∙ 9H 2 O, 1.24 ng/mL molybdic acid ammonium salt, 0.65 ng/mL NH 4 VO 3 , 0.13 ng/mL NiSO 4 ∙ 6H 2 O, 0.12 ng/mL SnCI 2 ), 10 μM Y-27632, and 2 μg/mL doxycycline (Takara). .. On day 4, the medium was changed to DMEM/F12 containing 0.5% N2 supplement, 0.5% B27 supplement lacking vitamin A, GlutaMAX, antibiotics, 200 μM ascorbic acid, 100 μM db-cAMP (Merck), 10 ng/mL IGF-I, 60 ng/mL T3, 0.1% trace elements cocktail solution, and 2 μg/mL doxycycline.

Derivative Assay:

Incubation:

Article Title: Dynamic regulation of origin firing factors links CDK activity to dormant origin activation.
Article Snippet: .. The resulting nuclei were resuspended in 200ul of a Click-it cocktail containing PBS, 2mM CuSO4, 2uM A647 Azide (Life Technologies), and 50mM ascorbic acid, incubated in the dark at room temperature for 90 minutes, washed and resuspended in a DNA staining solution containing 10ug/ml propdium iodide and 100ug/ml RNAse A prior to running on the analyzer (Cytoflex, Beckman-Coulter). ..

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